Exhibit 16.1
March 6, 2024
Securities and Exchange Commission
100 F Street, N.E.
Washington, DC 20549
Commissioners:
We have read the statements made by Grayscale Digital Large Cap Fund LLC under Item 4.01 of its Form 8-K dated March 6, 2024. We agree with the statements concerning our Firm in such Form 8-K; we are not in a position to agree or disagree with other statements of Grayscale Digital Large Cap Fund LLC contained therein.
Very truly yours,
/s/ Marcum llp
Marcum llp
