Bicycle Therapeutics (BCYC) SC 13DBICYCLE THERAPEUTICS / BAKER BROS. ADVISORS ownership change

Exhibit 99.1

JOINT FILING AGREEMENT

Pursuant to Rule 13d-1(k)(1) under the Securities Exchange Act of 1934, as amended, the undersigned hereby agree that only one statement containing the information required by Schedule 13D need be filed with respect to the ownership by each of the undersigned of the Ordinary Shares of Bicycle Therapeutics plc

This Agreement may be executed in any number of counterparts, each of which shall be deemed an original.

EXECUTED as of this 18th day of April 2024