Opthea Limited (OPT) F-1/ARegistration statement (foreign) (amended)

Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We consent to the use in this Amendment No. 2 to the Registration Statement on Form F-1 (File No. 333-249020) of our report dated September 1, 2020 relating to the financial statements of Opthea Limited. We also consent to the reference to us under the heading “Experts” in such Registration Statement.

/s/ Deloitte Touche Tohmatsu

Perth, Australia

October 15, 2020